Using a chemically defined monolayer neural differentiation

2018-10-20

Using a chemically defined, monolayer neural differentiation system (Lippmann et al., 2014), we determined that by temporally modulating Wnt/β-catenin and FGF signaling, hPSCs could be differentiated into pure SOX2+/Brachyury+ NMPs that were stable for 7 days. Over this period, the NMPs exhibited co

Introduction Adipose stromal progenitor cells

2018-10-20

Introduction Adipose stromal/progenitor Sitagliptin phosphate monohydrate (ASCs) are the major reservoir of adipocyte precursors and are crucial for adipose tissue renewal, homeostasis and expansion (Berry et al., 2016; Rodeheffer et al., 2008; Tang et al., 2008; Yamamoto et al., 2007). In white a

Acute and transient MF activation

2018-10-20

Acute and transient MF activation is part of the body’s normal wound healing program, but persistent MFs contribute to fibrosis by excessively producing and contracting collagenous extracellular matrix (ECM) into stiff scar tissue (Hinz et al., 2012). In turn, the stiffness of mature scar promotes m

In vitro we demonstrated that the DMR can drive luciferase

2018-10-20

In vitro, we demonstrated that the DMR can drive luciferase expression in three different cell lines (293T,COS-7, and HepG2) and markedly enhances reporter gene activity (10- to 15-fold increase) compared with basal activity obtained with either the SIX5 promoter, the entire region that surrounds th

Because Q SA and Q SC represent an

2018-10-20

Because Q3SA and Q3SC represent an isogenic pair of iPSC lines from the same individual but with contrasting ATM genotypes (ATM−/− versus ATM+/−, respectively), we examined gene expression differences using RNA sequencing (RNA-seq), evaluating matches at the transcript level. Relatively few mRNAs we

The impairment of cardiac differentiation resulting from Cit

2018-10-20

The impairment of cardiac differentiation resulting from Cited2 depletion at the onset of ESC differentiation may be due to a consequent downregulation of Brachyury and Mesp1 expression. The decrease of Isl1, Nkx2.5, Gata4, and Tbx5 expression that we observed in Cited2-depleted cells at D5 of diffe

Hello world!

2018-07-29